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imc analyses  (fluidigm)


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    Structured Review

    fluidigm imc analyses
    FIGURE 2 Summary of an analytic pipeline allowing the fine comparison of histochemical stain morphology and imaging mass cytometry <t>(IMC)</t> results within a single tissue section: example of an appendiceal sample. 1: The tissue section is produced based on a neutral formalin-fixed paraffin-embedded tissue sample and Alcian blue (AB) stained; 2: the AB-stained slide is digitalized; 3: the digital AB slide is used for the selection of the region of interest (ROI) for IMC analysis based on microscopic examination; 4: the ROI is positioned on the verso side of the AB-stained slide, which is used for IMC processing; 5: IMC acquisition of the ROI is performed; 6: the co-analysis of IMC data and digital AB slide allows the confrontation of morphological and immunophenotypic features until the single-cell level on the two images: note the exact overlapping and immunophenotyping between single cells within the Alcian blue (AB, CaseViewer, 3DHistech)) stain and IMC data (MCD Viewer, <t>Standard</t> <t>BioTools)</t> within the region of interest (ROI). (A) four neutrophile granulocytes with myeloperoxidase MPO positivity; (B) cytotoxic T cells CD8-positive within the appendiceal epithelium pan-keratin-positive; (C) three B cells CD20-positive and one neutrophile granulocyte MPO positive).
    Imc Analyses, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imc analyses/product/fluidigm
    Average 93 stars, based on 1523 article reviews
    imc analyses - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Coupling imaging mass cytometry with Alcian blue histochemical staining for a single-slide approach."

    Article Title: Coupling imaging mass cytometry with Alcian blue histochemical staining for a single-slide approach.

    Journal: Frontiers in immunology

    doi: 10.3389/fimmu.2024.1379154

    FIGURE 2 Summary of an analytic pipeline allowing the fine comparison of histochemical stain morphology and imaging mass cytometry (IMC) results within a single tissue section: example of an appendiceal sample. 1: The tissue section is produced based on a neutral formalin-fixed paraffin-embedded tissue sample and Alcian blue (AB) stained; 2: the AB-stained slide is digitalized; 3: the digital AB slide is used for the selection of the region of interest (ROI) for IMC analysis based on microscopic examination; 4: the ROI is positioned on the verso side of the AB-stained slide, which is used for IMC processing; 5: IMC acquisition of the ROI is performed; 6: the co-analysis of IMC data and digital AB slide allows the confrontation of morphological and immunophenotypic features until the single-cell level on the two images: note the exact overlapping and immunophenotyping between single cells within the Alcian blue (AB, CaseViewer, 3DHistech)) stain and IMC data (MCD Viewer, Standard BioTools) within the region of interest (ROI). (A) four neutrophile granulocytes with myeloperoxidase MPO positivity; (B) cytotoxic T cells CD8-positive within the appendiceal epithelium pan-keratin-positive; (C) three B cells CD20-positive and one neutrophile granulocyte MPO positive).
    Figure Legend Snippet: FIGURE 2 Summary of an analytic pipeline allowing the fine comparison of histochemical stain morphology and imaging mass cytometry (IMC) results within a single tissue section: example of an appendiceal sample. 1: The tissue section is produced based on a neutral formalin-fixed paraffin-embedded tissue sample and Alcian blue (AB) stained; 2: the AB-stained slide is digitalized; 3: the digital AB slide is used for the selection of the region of interest (ROI) for IMC analysis based on microscopic examination; 4: the ROI is positioned on the verso side of the AB-stained slide, which is used for IMC processing; 5: IMC acquisition of the ROI is performed; 6: the co-analysis of IMC data and digital AB slide allows the confrontation of morphological and immunophenotypic features until the single-cell level on the two images: note the exact overlapping and immunophenotyping between single cells within the Alcian blue (AB, CaseViewer, 3DHistech)) stain and IMC data (MCD Viewer, Standard BioTools) within the region of interest (ROI). (A) four neutrophile granulocytes with myeloperoxidase MPO positivity; (B) cytotoxic T cells CD8-positive within the appendiceal epithelium pan-keratin-positive; (C) three B cells CD20-positive and one neutrophile granulocyte MPO positive).

    Techniques Used: Comparison, Staining, Imaging, Mass Cytometry, Produced, Selection



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    imc analyses  (fluidigm)
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    fluidigm imc analyses
    FIGURE 2 Summary of an analytic pipeline allowing the fine comparison of histochemical stain morphology and imaging mass cytometry <t>(IMC)</t> results within a single tissue section: example of an appendiceal sample. 1: The tissue section is produced based on a neutral formalin-fixed paraffin-embedded tissue sample and Alcian blue (AB) stained; 2: the AB-stained slide is digitalized; 3: the digital AB slide is used for the selection of the region of interest (ROI) for IMC analysis based on microscopic examination; 4: the ROI is positioned on the verso side of the AB-stained slide, which is used for IMC processing; 5: IMC acquisition of the ROI is performed; 6: the co-analysis of IMC data and digital AB slide allows the confrontation of morphological and immunophenotypic features until the single-cell level on the two images: note the exact overlapping and immunophenotyping between single cells within the Alcian blue (AB, CaseViewer, 3DHistech)) stain and IMC data (MCD Viewer, <t>Standard</t> <t>BioTools)</t> within the region of interest (ROI). (A) four neutrophile granulocytes with myeloperoxidase MPO positivity; (B) cytotoxic T cells CD8-positive within the appendiceal epithelium pan-keratin-positive; (C) three B cells CD20-positive and one neutrophile granulocyte MPO positive).
    Imc Analyses, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/imc analyses/product/fluidigm
    Average 93 stars, based on 1 article reviews
    imc analyses - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    FIGURE 2 Summary of an analytic pipeline allowing the fine comparison of histochemical stain morphology and imaging mass cytometry (IMC) results within a single tissue section: example of an appendiceal sample. 1: The tissue section is produced based on a neutral formalin-fixed paraffin-embedded tissue sample and Alcian blue (AB) stained; 2: the AB-stained slide is digitalized; 3: the digital AB slide is used for the selection of the region of interest (ROI) for IMC analysis based on microscopic examination; 4: the ROI is positioned on the verso side of the AB-stained slide, which is used for IMC processing; 5: IMC acquisition of the ROI is performed; 6: the co-analysis of IMC data and digital AB slide allows the confrontation of morphological and immunophenotypic features until the single-cell level on the two images: note the exact overlapping and immunophenotyping between single cells within the Alcian blue (AB, CaseViewer, 3DHistech)) stain and IMC data (MCD Viewer, Standard BioTools) within the region of interest (ROI). (A) four neutrophile granulocytes with myeloperoxidase MPO positivity; (B) cytotoxic T cells CD8-positive within the appendiceal epithelium pan-keratin-positive; (C) three B cells CD20-positive and one neutrophile granulocyte MPO positive).

    Journal: Frontiers in immunology

    Article Title: Coupling imaging mass cytometry with Alcian blue histochemical staining for a single-slide approach.

    doi: 10.3389/fimmu.2024.1379154

    Figure Lengend Snippet: FIGURE 2 Summary of an analytic pipeline allowing the fine comparison of histochemical stain morphology and imaging mass cytometry (IMC) results within a single tissue section: example of an appendiceal sample. 1: The tissue section is produced based on a neutral formalin-fixed paraffin-embedded tissue sample and Alcian blue (AB) stained; 2: the AB-stained slide is digitalized; 3: the digital AB slide is used for the selection of the region of interest (ROI) for IMC analysis based on microscopic examination; 4: the ROI is positioned on the verso side of the AB-stained slide, which is used for IMC processing; 5: IMC acquisition of the ROI is performed; 6: the co-analysis of IMC data and digital AB slide allows the confrontation of morphological and immunophenotypic features until the single-cell level on the two images: note the exact overlapping and immunophenotyping between single cells within the Alcian blue (AB, CaseViewer, 3DHistech)) stain and IMC data (MCD Viewer, Standard BioTools) within the region of interest (ROI). (A) four neutrophile granulocytes with myeloperoxidase MPO positivity; (B) cytotoxic T cells CD8-positive within the appendiceal epithelium pan-keratin-positive; (C) three B cells CD20-positive and one neutrophile granulocyte MPO positive).

    Article Snippet: IMC analyses were conducted with the Hyperion® Imaging System technology (Standard BioTools) on tissue sections previously incubated (case #1) or not incubated (#2,#3,#4) with a pool of metal-coupled antibodies.

    Techniques: Comparison, Staining, Imaging, Mass Cytometry, Produced, Selection